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Application of two types of T. cruzi amastigotes of different virulence to ELISA for Chagas disease.

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dc.contributor.author Rojas de Arias, Gladys Antonieta
dc.contributor.author Maldonado, Marisel
dc.contributor.author Ichinose, Yoshio
dc.contributor.author Samudio, Margarita
dc.contributor.author Sakamo, Makoto
dc.contributor.author Moreno Azorero, Ricardo
dc.contributor.author Kanbara, Hiroji
dc.date.accessioned 2019-12-21T19:56:00Z
dc.date.available 2019-12-21T19:56:00Z
dc.date.issued 1990
dc.identifier.uri http://dspace.conacyt.gov.py/xmlui/handle/123456789/42307
dc.description.abstract Trypanosoma cruzi amastigotes derived from fibroblast cultures and from cell-free liquid medium were comparatively evaluated as solid-phase antigens of ELISA for Chagas' disease. Amastigotes were obtained from the high- and low-virulent clones of the Tulahuen strain and from the G-1 strain. They were sonicated and directly applied into wells of a microplate to use insoluble particles as antigens. Fifty-one sera from patients of Chagas' disease including one acute case (with Romana's sign), 10 sera from patients of cutaneous leishmaniasis including one serum of mixed infection with Chagas' disease and 10 sera from patients of toxoplasmosis including three of mixed infection with either Chagas' disease or leishmaniasis were examined. All amastigotes from different sources and from different clones or strains showed considerable correlation with each other. Slightly higher sensitivity was found in amastigotes of high-virulent clones and slightly higher specificity in those from cell-free culture. One acute case was detected as positive by these methods although it was negative by the usual ELISA using epimastigotes Application of two types of Trypanosoma cruzi amastigotes of different virulence to ELISA for Chagas' disease. (PDF Download Available). Available from: https://www.researchgate.net/publication/40231554_Application_of_two_types_of_Trypanosoma_cruzi_amastigotes_of_different_virulence_to_ELISA_for_Chagas%27_disease [accessed May 2, 2017].
dc.format application/pdf
dc.relation.uri http://dspace.conacyt.gov.py/jspui/bitstream/123456789/42307/1/1990 application of two types of t. cruzi amastigotes of different virulence to elisa for chagas disease.pdf
dc.rights Japanese Society of Tropical Medicine
dc.source Journal of Tropical Medicine and Hygiene
dc.subject.mesh Chagas disease
dc.subject.mesh ELISA
dc.subject.mesh Tripanosoma cruzi
dc.title Application of two types of T. cruzi amastigotes of different virulence to ELISA for Chagas disease.
dc.type Artículo
dcterms.bibliographicCitation Journal of Tropical Medicine and Hygiene 18(4), 325 - 332. 1990
dc.contributor.identifier 1261551
dc.contributor.identifier 574675
dc.contributor.identifier 998549
dc.directorio.catalogador arv
dc.identifier.doi 10.2149/tmh1973.18.325
dc.source.number 4
dc.source.pages 325 - 332
dc.source.volume 18

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